Journal: eLife
Article Title: Articular cartilage corefucosylation regulates tissue resilience in osteoarthritis
doi: 10.7554/eLife.92275
Figure Lengend Snippet: ( A ) Time course of intra-articular α-mannosidase injection and unloading. Red arrows indicate the one-shot, intra-articular injection that was performed once a week. ( B ) Histological evaluation of knee articular cartilage 4 weeks after intermittent α-mannosidase infusion and 16 weeks after load removal. Scale bars, 50 μm. ( C ) Representative macroscopic assessment of the articular cartilage using mannosidase concentration. Scale bar, 1 cm. ( D ) Evaluation of the collagen orientation of reparative tissues at 4 weeks postoperatively. Sections stained with HE was viewed under a polarized light microscope at multiple angles (0°, 45°, and 90°). Scale bar, 200 μm. ( E ) OARSI scores at 4 weeks postoperatively on sections from the knee joint (n=6). ( F ) Tissue degeneration scores at each time point are based on mannosidase concentration. Data are shown as mean ± standard deviation. *p<0.05, **p<0.01 versus the saline group in ( E ), and versus 4 weeks in ( F ). In ( E ), the Welch t-test was used for statistical analysis. In ( F ), n=6 rabbits per group. One-way analysis of variance with Tukey’s multiple comparison test was used to perform statistical analysis. HE, hematoxylin and eosin; OARSI, Osteoarthritis Research Society International; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.
Article Snippet: To evaluate the collagen orientation of the repaired tissue at 16 weeks postoperatively, a supplemental evaluation using a polarized light microscope (PLM; ECLIPSE E600 POL; Nikon, Tokyo, Japan) was conducted ( ).
Techniques: Injection, Concentration Assay, Staining, Light Microscopy, Standard Deviation, Saline, Comparison, TUNEL Assay